ESTABLISHED BONE-RELATED CELL LINES (under construction)
Osteoblast lineage
MC3T3-E1 (mouse)(1) osteoblastic cell line otained by cloning of clels isolated by collagenase digestion of newborn mouse calvariae.
MBA-15 osteoblastic line dervied from mouse bone marrow stroma (2) MBA15.33, MBA 15.4 (early stage subclones) MBA 15.6 (later stage subclones)
MLO-Y4 ( murine long bone osteocyte Y4 ) osteocyte-like cells (3) isolated from transgenic mice with SV40 large T-antigen oncogene under osteocalcin promoter.D1 ORL UVA [D1] clone from multipotent mouse bone marrow stromal precursor that can differentiate into osteocytes, chondrocytes and adipocytes.
ROS 17/2.8 Rat osteosarcoma cell line representative of late stage osteoblastic cell.
UMR 106.06 Rat osteosarcoma cell line. osteoblast-like cell line
RCT-1 Rat virally immortalized cell line
RCT-2 Rat virally immortalized cell line
HOS/TE-85 Human osteosarcoma cell line
MG-63 Human osteosarcoma cell line
SaOS-2 Human osteosarcoma cell line
HOBIT (Human osteoblast-like Initial Transfectant) cells. Human virally immortalized cell line
MBA-15 osteoblastic line dervied from mouse bone marrow stroma (2) MBA15.33, MBA 15.4 (early stage subclones) MBA 15.6 (later stage subclones)
MLO-Y4 ( murine long bone osteocyte Y4 ) osteocyte-like cells (3) isolated from transgenic mice with SV40 large T-antigen oncogene under osteocalcin promoter.D1 ORL UVA [D1] clone from multipotent mouse bone marrow stromal precursor that can differentiate into osteocytes, chondrocytes and adipocytes.
ROS 17/2.8 Rat osteosarcoma cell line representative of late stage osteoblastic cell.
UMR 106.06 Rat osteosarcoma cell line. osteoblast-like cell line
RCT-1 Rat virally immortalized cell line
RCT-2 Rat virally immortalized cell line
HOS/TE-85 Human osteosarcoma cell line
MG-63 Human osteosarcoma cell line
SaOS-2 Human osteosarcoma cell line
HOBIT (Human osteoblast-like Initial Transfectant) cells. Human virally immortalized cell line
Osteoclast lineage
RAW264.7 Mouse Osteoclast precursor-like cells
Chondrocyte lineage
MC615 cloned immortalized mouse chondrocytic cells (4)
H4 immortalized murine chondrocyte cell line obtained from mature arictular chondrocytes (5)
MM-Sv/HP-2 murine adenovirus infected femoral head cartilage cells (6)
CHON-001 human normal cartilage fibroblast immortablized with hTERT
CHON-002 human normal cartilage fibroblast immortablized with hTERT
H4 immortalized murine chondrocyte cell line obtained from mature arictular chondrocytes (5)
MM-Sv/HP-2 murine adenovirus infected femoral head cartilage cells (6)
CHON-001 human normal cartilage fibroblast immortablized with hTERT
CHON-002 human normal cartilage fibroblast immortablized with hTERT
References
General:Methods in bone biology
(1) Kodama, H., Amagai, Y., Sudo, H. et al. (1982) Establishment of clonal osteogenic cell line from newborn mouse calvaria. Jpanese Journal of Oral Biology 23, 899-904.
(2) Benayahu, D., Kletter, Y., Zipori, D. and Wientroub, S. (1989) Bone marrow-dervied stromal cell line expressing osteoblastic phenotype in vitro and osteogenic capacity in vivo. Journal of Cellular Physiology 140, 1-7.
(3) Kato, Y., Windle, J., Koop, B. Mundy, G., Bonewarld, L. (1997) Establishment of an osteocyte-like cell line, MLO-Y4. J Bone Miner Res. 12, 2014-23.
(4) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1218864/pdf/9581563.pdf
(5) H.M. van Beuningen, R. Stoop, P. Buma, N. Takahashi, P.M. van der Kraan and W.B. van den Berg, Phenotypic differences in murine chondrocyte cell lines derived from mature articular cartilage. Osteoarthritis Cartilage, 10 12 (2002), pp. 977–986. http://www.sciencedirect.com/science?_ob=MiamiImageURL&_cid=272474&_user=994540&_pii=S1063458402908551&_check=y&_coverDate=2002-12-01&view=c&wchp=dGLzVlV-zSkWb&md5=637b72ee6f917ecaa8396ff31076347b/1-s2.0-S1063458402908551-main.pdf
(6) http://onlinelibrary.wiley.com/doi/10.1002/jcp.21282/abstract